Journal Article

<i>TONSOKU</i> is Expressed in S Phase of the Cell Cycle and its Defect Delays Cell Cycle Progression in <i>Arabidopsis</i>

Takamasa Suzuki, Sakiko Nakajima, Soichi Inagaki, Makiko Hirano-Nakakita, Ken Matsuoka, Taku Demura, Hiroo Fukuda, Atsushi Morikami and Kenzo Nakamura

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 46, issue 5, pages 736-742
Published in print May 2005 | ISSN: 0032-0781
Published online May 2005 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pci082
TONSOKU is Expressed in S Phase of the Cell Cycle and its Defect Delays Cell Cycle Progression in Arabidopsis

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TONSOKU(TSK)/MGOUN3/BRUSHY1 of Arabidopsis thaliana encodes a nuclear leucine–glycine–aspargine (LGN) domain protein implicated to be involved in genome maintenance, and mutants with defects in TSK show a fasciated stem with disorganized meristem structures. We identified a homolog of TSK from tobacco BY-2 cells (NtTSK), which showed high sequence conservation both in the LGN domain and in leucine-rich repeats with AtTSK. The NtTSK gene was expressed during S phase of the cell cycle in tobacco BY-2 cells highly synchronized for cell division. The tsk mutants of Arabidopsis contained an increased proportion of cells with 4C nuclei and cells expressing cyclin B1 compared with the wild type. These results suggest that TSK is required during the cell cycle and defects of TSK cause the arrest of cell cycle progression at G2/M phase.

Keywords: Arabidopsis; Cell cycle; G2 checkpoint; Genome maintenance; TONSOKU/MGOUN3/BRUSHY1; CAF-I, chromatin assembly factor-I; CDK, cyclin-dependent kinase; EST, expressed sequence tag; GUS, β-glucuronidase; LGN, leucine–glycine–asparagine; LRR, leucine-rich repeat; PCNA, proliferating cell nuclear antigen; RAM, root apical meristem; RT–PCR, reverse transcription–polymerase chain reaction; SAM, shoot apical meristem

Journal Article.  4233 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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