Journal Article

Evidence for the Plasma Membrane Localization of Al-activated Malate Transporter (ALMT1)

Mineo Yamaguchi, Takayuki Sasaki, Mayandi Sivaguru, Yoko Yamamoto, Hiroki Osawa, Sung Ju Ahn and Hideaki Matsumoto

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 46, issue 5, pages 812-816
Published in print May 2005 | ISSN: 0032-0781
Published online May 2005 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pci083
Evidence for the Plasma Membrane Localization of Al-activated Malate Transporter (ALMT1)

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Aluminum (Al)-activated malate transporter (ALMT1) was recently identified from wheat (Triticum aestivum). Heterologous expression of ALMT1 led to higher malate exudation that is associated with enhanced Al tolerance in transgenic plants. Here, we show the first direct evidence that ALMT1 is localized in the plasma membrane of Al-tolerant wheat. Phase partitioning experiments showed that this transporter was associated with the plasma membrane fraction. ALMT1 was detected in an Al-tolerant wheat line even without Al treatments. Analysis of transient expression of ALMT1::green fluorescent protein (GFP) in onion and tobacco cells further confirmed this ALMT1 localization.

Keywords: Aluminum stress; Aluminum-activated malate transporter; Green fluorescent protein; Plasma membrane; Triticum aestivum L.; ALMT1, aluminum-activated malate transporter; BiP, binding protein; CaMV, cauliflower mosaic virus; GFP, green fluorescent protein; PM-ATPase, plasma membrane H+-ATPase; PPase, H+-pyrophosphatase

Journal Article.  3032 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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