Journal Article

Peroxisomal Localization of a Myosin XI Isoform in <i>Arabidopsis thaliana</i>

Kohsuke Hashimoto, Hisako Igarashi, Shoji Mano, Mikio Nishimura, Teruo Shimmen and Etsuo Yokota

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 46, issue 5, pages 782-789
Published in print May 2005 | ISSN: 0032-0781
Published online May 2005 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pci085
Peroxisomal Localization of a Myosin XI Isoform in Arabidopsis thaliana

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  • Molecular and Cell Biology
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The genome of Arabidopsis thaliana contains 13 myosin XI isoforms. Here we prepared a specific antibody against a peptide that mimics a unique C-terminal region from the myosin XI isoform, MYA2. The resulting antibody was used to demonstrate that MYA2 in Arabidopsis protein extracts co-sedimented with actin filaments and dissociated from the filaments with ATP treatment. Immunolocalization studies showed that MYA2 co-localized predominantly with actin filaments in clustered punctuate dots in leaf epidermal cells, root hair cells and suspension-cultured cells. In a transgenic plant in which peroxisomes are labeled with green fluorescent protein, some MYA2 signals were localized on peroxisomes in an actin-dependent manner. We propose that the peroxisome is one of the cargos translocated by MYA2 on actin filaments.

Keywords: Actin; Arabidopsis; MYA2; Myosin XI; Peroxisome; BSA, bovine serum albumin; CBB, Coomassie brilliant blue; GFP, green fluorescent protein; LB, latrunculin B; PBS, phosphate-buffered saline; RT–PCR, reverse transcription–polymerase chain reaction

Journal Article.  5163 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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