Journal Article

A Novel Receptor Kinase Involved in Jasmonate-mediated Wound and Phytochrome Signaling in Maize Coleoptiles

Guozhen He, Yutaka Tarui and Moritoshi Iino

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 46, issue 6, pages 870-883
Published in print June 2005 | ISSN: 0032-0781
Published online June 2005 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pci092
A Novel Receptor Kinase Involved in Jasmonate-mediated Wound and Phytochrome Signaling in Maize Coleoptiles

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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We identified a gene of maize (Zea mays L.) that is transcriptionally activated in decapitated coleoptiles. The amino acid sequence deduced from its full-length cDNA indicated that the identified gene encodes a novel leucine-rich-repeat receptor-like kinase. The gene is named WOUND-RESPONSIVE AND PHYTOCHROME-REGULATED KINASE1 (WPK1) based on the findings of this study. Database searches revealed two and three homologs of WPK1 for Arabidopsis thaliana and rice, respectively. These homologs occurred along with WPK1 on a phylogenetic branch separated from all reported receptor kinases. We uncovered that the level of WPK1 transcripts is up-regulated rapidly and transiently in response to wounding and red light. The response to red light was reversible by far-red light, indicating that it is mediated by phytochrome. Applied jasmonic acid activated the expression of WPK1, while ethylene, salicylic acid and abscisic acid had no such effect. These results strongly suggested that WPK1 is a component of the jasmonate-mediated signaling that participates in both wound-induced defensive and phytochrome-mediated photomorphogenetic responses. Furthermore, it was found that both wounding and red light up-regulate the transcript level of ZmAOS, a gene for the jasmonate biosynthesis enzyme allene oxide synthase, and that auxin inhibits the expression of WPK1 but not of ZmAOS. We present a model of jasmonate-mediated signaling to explain the results obtained.

Keywords: Coleoptiles; Jasmonate; Phytochrome; Receptor kinase; Wound signaling; Zea mays L.; AOS, allene oxide synthase; ECL, enhanced chemiluminescence; FR, far-red light; JA, jasmonic acid; LRR, leucine-rich repeat; MAP, mitogen-activated protein; 1-NAA, 1-naphthyl acetic acid; NPA, N-1-naphthylphthalamic acid; R, red light; RT–PCR, reverse transcription–polymerase chain reaction; SA, salicylic acid; WPK1, WOUND-RESPONSIVE AND PHYTOCHROME-REGULATED KINASE1

Journal Article.  10399 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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