Journal Article

The <i>Arabidopsis</i> Gene <i>CAD1</i> Controls Programmed Cell Death in the Plant Immune System and Encodes a Protein Containing a MACPF Domain

Chizuko Morita-Yamamuro, Tomokazu Tsutsui, Masanao Sato, Hirofumi Yoshioka, Masanori Tamaoki, Daisuke Ogawa, Hideyuki Matsuura, Teruhiko Yoshihara, Akira Ikeda, Ichiro Uyeda and Junji Yamaguchi

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 46, issue 6, pages 902-912
Published in print June 2005 | ISSN: 0032-0781
Published online June 2005 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pci095
The Arabidopsis Gene CAD1 Controls Programmed Cell Death in the Plant Immune System and Encodes a Protein Containing a MACPF Domain

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  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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To clarify the processes involved in plant immunity, we have isolated and characterized a single recessive Arabidopsis mutant, cad1 (constitutively activated cell death 1), which shows a phenotype that mimics the lesions seen in the hypersensitive response (HR). This mutant shows spontaneously activated expression of pathogenesis-related (PR) genes, and leading to a 32-fold increase in salicylic acid (SA). Inoculation of cad1 mutant plants with Pseudomonas syringae pv tomato DC3000 shows that the cad1 mutation results in the restriction of bacterial growth. Cloning of CAD1 reveals that this gene encodes a protein containing a domain with significant homology to the MACPF (membrane attack complex and perforin) domain of complement components and perforin proteins that are involved in innate immunity in animals. Furthermore, cell death is suppressed in transgenic cad1 plants expressing nahG, which encodes an SA-degrading enzyme. We therefore conclude that the CAD1 protein negatively controls the SA-mediated pathway of programmed cell death in plant immunity.

Keywords: Hypersensitive response; Membrane attack complex and perforin; Plant immunity; Programmed cell death; Salicylic acid; CFP, cyan fluorescent protein; ClYVV, clover yellow vein virus; GFP, green fluorescent protein; GUS, β-glucuronidase; HR, hypersensitive response; MACPF, membrane attack complex and perforin; nahG, salicylate hydroxylase; NPR1, NONEXPRESSOR OF PR GENES 1; PCD, programmed cell death; PCR, polymerase chain reaction; PR genes, pathogenesis-related genes; SA, salicylic acid; TIR, Toll/interleukin-1 receptor

Journal Article.  6500 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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