Journal Article

Regulation of <i>CAPRICE</i> Transcription by MYB Proteins for Root Epidermis Differentiation in <i>Arabidopsis</i>

Yoshihiro Koshino-Kimura, Takuji Wada, Tatsuhiko Tachibana, Ryuji Tsugeki, Sumie Ishiguro and Kiyotaka Okada

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 46, issue 6, pages 817-826
Published in print June 2005 | ISSN: 0032-0781
Published online June 2005 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pci096
Regulation of CAPRICE Transcription by MYB Proteins for Root Epidermis Differentiation in Arabidopsis

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  • Molecular and Cell Biology
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Epidermal cell differentiation in Arabidopsis root is studied as a model system for understanding cell fate specification. Two types of MYB-related transcription factors are involved in this cell differentiation. One of these, CAPRICE (CPC), encoding an R3-type MYB protein, is a positive regulator of hair cell differentiation and is preferentially transcribed in hairless cells. We analyzed the regulatory mechanism of CPC transcription. Deletion analyses of the CPC promoter revealed that hairless cell-specific transcription of the CPC gene required a 69 bp sequence, and a tandem repeat of this region was sufficient for its expression in epidermis. This region includes two MYB-binding sites, and the epidermis-specific transcription of CPC was abolished when base substitutions were introduced in these sites. We showed by gel mobility shift experiments and by yeast one-hybrid assay that WEREWOLF (WER), which is an R2R3-type MYB protein, directly binds to this region. We showed that WER also binds to the GL2 promoter region, indicating that WER directly regulates CPC and GL2 transcription by binding to their promoter regions.

Keywords: CAPRICE; Cell differentiation; DNA binding; MYB protein; Transcription factor; WEREWOLF; bHLH, basic helix–loop–helix; CPC, CAPRICE; DTT, dithiothreitol; GL, GLABRA; GST, glutathione S-transferase; GUS, β-glucuronidase; EGL, ENHANCER OF GLABRA; TTG, TRANSPARENT TESTA GLABRA; WER, WEREWOLF

Journal Article.  7183 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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