Journal Article

Nitric Oxide is Involved in Methyl Jasmonate-induced Defense Responses and Secondary Metabolism Activities of <i>Taxus</i> Cells

Jian Wen Wang and Jian Yong Wu

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 46, issue 6, pages 923-930
Published in print June 2005 | ISSN: 0032-0781
Published online June 2005 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pci098
Nitric Oxide is Involved in Methyl Jasmonate-induced Defense Responses and Secondary Metabolism Activities of Taxus Cells

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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Methyl jasmonate (MeJA), a methyl ester of jasmonic acid (JA), is a well-established signal molecule in plant defense responses and an effective inducer of secondary metabolite accumulation in plant cell cultures such as the valuable anticancer diterpenoid taxol (paclitaxel) in Taxus spp. This work examines the involvement of nitric oxide (NO) in MeJA-induced plant defense responses and secondary metabolism in Taxus chinensis cell cultures. Exogenously supplied MeJA at 100 µM induced rapid production of NO in the Taxus cell cultures, reaching a maximum within 6 h of MeJA supply. Several other responses occurred concomitantly, including the production of hydrogen peroxide (H2O2), and the increases in intracellular malondialdehyde (MDA) content, lipoxygenase (LOX) and phenylalanine ammonium-lyase (PAL) activities. The MeJA-induced H2O2 production was suppressed by an NO donor, sodium nitroprusside (SNP), but enhanced by NO inhibitors, N ω-nitro-l-arginine (l-NNA) and 2-phenyl-4,4,5,5-tetramethyl-imidazoline-1-oxyl-3-oxide (PTIO). In contrast, the MeJA-induced MDA, LOX and PAL were all enhanced by the NO donor but suppressed by the NO inhibitors. The NO inhibitors also suppressed MeJA-induced taxol accumulation. These results are suggestive of a role for NO as a signal element for activating the MeJA-induced defense responses and secondary metabolism activities of plant cells.

Keywords: Methyl jasmonate; Nitric oxide; Oxidative responses; Paclitaxel production; Phenylalanine ammonium-lyase; Taxus cell culture; CHS, chalcone synthase; DAF-2 DA, 4,5-diaminofluorescein diacetate; IBU, ibuprofen; JA, jasmonic acid; LOX, lipoxygenase; MDA, malondialdehyde; MeJA, methyl jasmonate; l-NNA, Nω-nitro-l-arginine; NO, nitric oxide; NOS, nitric oxide synthase; PAL, phenylalanine ammonium lyase; PTIO, 2-phenyl-4,4,5,5-tetramethyl-imidazoline-1-oxyl-3-oxide; ROS, reactive oxygen species; SNP, sodium nitroprusside; TBA, thiobarbituric acid; TCA, trichloroacetic acid

Journal Article.  5437 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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