Journal Article

A Peroxiredoxin Q Homolog from Gentians is Involved in Both Resistance Against Fungal Disease and Oxidative Stress

Akinori Kiba, Masahiro Nishihara, Nobue Tsukatani, Takashi Nakatsuka, Yoshiaki Kato and Saburo Yamamura

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 46, issue 6, pages 1007-1015
Published in print June 2005 | ISSN: 0032-0781
Published online June 2005 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pci109
A Peroxiredoxin Q Homolog from Gentians is Involved in Both Resistance Against Fungal Disease and Oxidative Stress

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An antifungal protein (GtAFP1) showing antimicrobial activity against phytopathogenic fungi was purified from leaves of Gentiana triflora. The deduced amino acid sequence of the cDNA of the corresponding gene, GtAFP1, showed 94, 75, 72 and 63% amino acid identities with peroxiredoxin Q from Populus balsamifera × P. deltoides subsp. trichocarpa, Sedum lineare, Suaeda maritima and Arabidopsis thaliana, respectively. The GtAFP1 gene is suggested to be present in the genome in one to two copies and was expressed in the leaves, roots and stems. Expression of GtAFP1 was induced by treatment with salicylic acid, but not methyl jasmonate. Recombinant GtAFP1 protein showed not only antifungal activity but also thioredoxin-dependent peroxidase activity. Overexpression of GtAFP1 in tobacco plants improved tolerance not only against fungal diseases but also against oxidative stress. These results indicate that GtAFP1 might act as a disease and oxidative stress defensive gene in plants and could be useful for engineering stress-resistant plants.

Keywords: Antifungal protein; Gentiana triflora; Peroxiredoxin Q; Recombinant protein; Thioredoxin peroxidase; Transgenic tobacco; Bcp, bacterioferritin co-migratory protein; CaMV, cauliflower mosaic virus; DIG, digoxigenin; GtAFP1, Gentiana triflora antifungal protein 1; JA, methyl jasmonate; LTP, lipid transfer protein; ORF, open reading frame; RACE, rapid amplification of cDNA ends; ROI, reactive oxygen intermediate; RT–PCR, reverse transcription–polymerase chain reaction; SA, salicylic acid; TFA, trifluoroacetic acid; Trx, thioredoxin

Journal Article.  5845 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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