Journal Article

A Resource of 5,814 <i>Dissociation</i> Transposon-tagged and Sequence-indexed Lines of <i>Arabidopsis</i> Transposed from Start Loci on Chromosome 5

Takuya Ito, Reiko Motohashi, Takashi Kuromori, Yoshiteru Noutoshi, Motoaki Seki, Asako Kamiya, Saho Mizukado, Tetsuya Sakurai and Kazuo Shinozaki

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 46, issue 7, pages 1149-1153
Published in print July 2005 | ISSN: 0032-0781
Published online July 2005 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pci112
A Resource of 5,814 Dissociation Transposon-tagged and Sequence-indexed Lines of Arabidopsis Transposed from Start Loci on Chromosome 5

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We report here the generation of an additional collection of Dissociation (Ds) transposon-tagged, sequence-indexed lines of Arabidopsis thaliana. Our RIKEN Ds insertion collection now totals 17,668 lines. Our collection has preferential insertions in chromosomes 1 and 5, because Ds was transposed from start loci on those chromosomes (11,854 and 5,814 lines, respectively). We describe here features of the latter 5,814 lines. The former 11,854 lines have been described previously. We have created a searchable database of the insertion sites and mutated genes (http://rarge.gsc.riken.jp/), and are depositing these lines in the RIKEN BioResource Center (http://www.brc.riken.go.jp/lab/epd/Eng/). Our collection of these mutants will contribute to progress in functional genomics of plants.

Keywords: Ac/Ds; Arabidopsis thaliana; Functional genomics; ABRC, Arabidopsis Biological Resource Center; Ac, Activator; Ds, Dissociation

Journal Article.  2729 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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