Journal Article

Caffeine Inhibits Callose Deposition in the Cell Plate and the Depolymerization of Microtubules in the Central Region of the Phragmoplast

Hiroki Yasuhara

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 46, issue 7, pages 1083-1092
Published in print July 2005 | ISSN: 0032-0781
Published online July 2005 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pci121
Caffeine Inhibits Callose Deposition in the Cell Plate and the Depolymerization of Microtubules in the Central Region of the Phragmoplast

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Treatment of tobacco BY-2 cells with 10 mM caffeine that was started after the cells had entered the mitotic phase did not completely inhibit the deposition of callose in the cell plate and allowed the centrifugal redistribution of phragmoplast microtubules. On the other hand, when treatment with caffeine was started before the cells entered the mitotic phase, the deposition of callose was completely inhibited and the redistribution of phragmoplast microtubules was also inhibited. As the inhibition of redistribution of phragmoplast microtubules seems to be caused by the inhibition of depolymerization of microtubules at the central region of the phragmoplast, these results strongly suggest that the deposition of callose in the cell plate is tightly linked with the depolymerization of phragmoplast microtubules. Callose deposition was observed in phragmoplasts isolated from caffeine-treated cells as well as in those isolated from non-caffeine-treated cells, and caffeine did not inhibit callose synthesis in isolated phragmoplast, indicating that caffeine neither inhibits the accumulation of callose synthase at the equatorial regions of the phragmoplast nor arrests callose synthase itself.

Keywords: Caffeine; Callose; Cell plate; Cytokinesis; Phragmoplast; Tobacco BY-2 cells; BFA, brefeldin A; DAPI, 4′,6′-diamino-2-phenylindole; MAPK, mitogen-activated protein kinase

Journal Article.  5720 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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