Journal Article

Expression of Two Genes Encoding Gibberellin 2- and 3-oxidases in Developing Seeds of <i>Phaseolus</i> <i>coccineus</i>

Cinzia Solfanelli, Francesca Ceron, Fabio Paolicchi, Lucia Giorgetti, Chiara Geri, Nello Ceccarelli, Yuji Kamiya and Piero Picciarelli

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 46, issue 7, pages 1116-1124
Published in print July 2005 | ISSN: 0032-0781
Published online July 2005 | e-ISSN: 1471-9053 | DOI: https://dx.doi.org/10.1093/pcp/pci124
Expression of Two Genes Encoding Gibberellin 2- and 3-oxidases in Developing Seeds of Phaseolus coccineus

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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We have isolated PcGA3ox1, a cDNA clone from developing runner bean (Phaseolus coccineus) seeds that shows significant amino acid homology with the gibberellin (GA) 3-oxidases. A recombinant fusion protein of PcGA3ox1 converted GA20 and GA9 to GA1 and GA4, respectively. In situ hybridization results showed that transcripts of this gene accumulate specifically within the suspensor of globular-stage embryos. PcGA3ox1 mRNA begins to accumulate in the epidermal cells of the embryo proper and is also detectable in the endosperm during the transition from globular- to heart-stage embryos. PcGA3ox1 transcripts were localized exclusively in the cotyledons from the early cotyledonary stage up to the cotyledonary stage. Transcripts of the previously cloned GA 2-oxidase (PcGA2ox1) from developing seeds of runner bean were found primarily within the suspensor neck region from the late globular stage up to the heart stage. PcGA2ox1 mRNA was detectable in the whole suspensor from the early cotyledonary stage, and was found in the inner layer of integuments at the cotyledonary stage. Soluble enzyme preparations made from suspensors and embryos at two stages of embryogenesis (the heart and cotyledonary stages) were incubated with [14C]GA20 and [14C]GA1. Only young suspensor preparations converted GA20 to GA1 and GA5. Both suspensor preparations converted GA1 to GA8. Both embryo preparations converted GA20 to GA1, but were unable to convert GA1 to GA8.

Keywords: Developing seed; GA 2-oxidase; GA 3-oxidase; Gibberellin; Runner bean; GA, gibberellin; GC-MS, gas chromatography–mass spectrometry; 2ODD, 2-oxoglutarate-dependent dioxygenase

Journal Article.  7572 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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