Journal Article

Transcriptome Profiling of Vertical Stem Segments Provides Insights into the Genetic Regulation of Secondary Growth in Hybrid Aspen Trees

Constantinos Prassinos, Jae-Heung Ko, Jaemo Yang and Kyung-Hwan Han

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 46, issue 8, pages 1213-1225
Published in print August 2005 | ISSN: 0032-0781
Published online August 2005 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pci130
Transcriptome Profiling of Vertical Stem Segments Provides Insights into the Genetic Regulation of Secondary Growth in Hybrid Aspen Trees

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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In order to better understand the genetic regulation of secondary growth in hybrid aspen (Populus tremula L.×P. alba L.), we carried out a series of cDNA-amplified fragment length polymorphism (AFLP)-based transcriptome analyses in vertical stem segments that represent a gradient of developmental stages with regard to secondary growth. This approach allowed us to screen >80% of the transcriptome expressed in six samples and identify genes differentially expressed with the progress of secondary growth, in a tissue-specific manner. Of the 76,800 transcript-derived fragments (TDFs) analyzed, 271 TDFs were selected and sequenced based on their differential expression patterns. Many of the xylem-up-regulated genes were involved in cell wall and lignin biosynthesis, while the bark-up-regulated genes had diverse functional roles. About 25% of the xylem-up-regulated TDFs analyzed were involved in the phenylpropanoid biosynthesis pathway, which produces the cell wall polymer lignin and various wood extractives. In addition, many of the TDFs showing secondary xylem-specific expression were annotated as genes not previously reported in Populus, including novel cell death proteins, cytoskeleton-interacting proteins, transporters and putative transcription factors.

Keywords: cDNA-AFLP; Populus; Secondary growth; Stem; Wood formation; AFLP, amplified fragment length polymorphism; HD-Zip, homeo-box domain leucine zipper; L, leaf sample; PCD, programmed cell death; PG, primary growth sample; SAM, S-adenosyl-l-methionine; SG, secondary growth sample; SXF, secondary xylem-forming; TDF, transcript-derived fragment; TS, transition zone sample; X, xylem sample

Journal Article.  7908 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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