Journal Article

Temperature and Light Modulate the <i>trans</i>-Δ<sup>3</sup>-Hexadecenoic Acid Content of Phosphatidylglycerol: Light-harvesting Complex II Organization and Non-photochemical Quenching

Gordon R. Gray, Alexander G. Ivanov, Marianna Król, John P. Williams, Mobashoher U. Kahn, Elizabeth G. Myscich and Norman P. A. Huner

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 46, issue 8, pages 1272-1282
Published in print August 2005 | ISSN: 0032-0781
Published online August 2005 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pci136
Temperature and Light Modulate the trans-Δ3-Hexadecenoic Acid Content of Phosphatidylglycerol: Light-harvesting Complex II Organization and Non-photochemical Quenching

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The interaction of light and temperature in the modulation of the trans3-hexadecenoic acid (trans-16:1) content of phosphatidylglycerol (PG) in winter rye (Secale cereale L.) was assessed and related to the organization of light-harvesting complex II (LHCII). Increasing the growth irradiance from 50 to 800 µmol m–2 s–1 at 20°C resulted in a 1.8-fold increase in the trans-16:1 content in PG which favoured a greater preponderance of oligomeric LHCII, measured in vitro as the ratio of oligomer : monomer. Similar irradiance-dependent increases were observed during growth at 5°C; however, 1.4-fold lower trans-16:1 contents and lower LHCII oligomer : monomer ratios were observed compared with growth at 20°C and the same irradiance. These trends were also observed under natural field conditions. Thus, the accumulation of trans-16:1, as well as the organization of LHCII are modulated by both growth irradiance and growth temperature in an independent but additive manner. We also examined how changes in the supramolecular organization of LHCII affected the capacity for non-photochemical quenching (qN) and photoprotection via antenna quenching (qO). While qO was positively correlated with qN, there was no correlation with either LHCII organization or xanthophyll cycle activity under the steady-state growth conditions examined.

Keywords: Antenna quenching; Light-harvesting complex II; Non-photochemical quenching; Phosphatidylglycerol; Supramolecular organization; trans-Δ3-hexadecenoic acid; A, antheraxanthin; EPS, epoxidation state; LHCII, light-harvesting complex II; Lhcb, Chla/b-binding light-harvesting polypeptides associated with PSII; Lut, lutein; Neo, neoxanthin; PG, phosphatidylglycerol; QA, first stable electron acceptor of PSII; qE, energy-dependent component of non-photochemical quenching; qN, coefficient of non-photochemical quenching; qO, quenching coefficient of basal fluorescence (antenna quenching); 1 − qP, relative reduction state of QA (PSII excitation pressure); trans-16:1, trans-Δ3-hexadecenoic acid; V, violaxanthin; Z, zeaxanthin

Journal Article.  9234 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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