Journal Article

Homoglutathione Confers Tolerance to Acifluorfen in Transgenic Tobacco Plants Expressing Soybean Homoglutathione Synthetase

Akifumi Sugiyama and Jiro Sekiya

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 46, issue 8, pages 1428-1432
Published in print August 2005 | ISSN: 0032-0781
Published online August 2005 | e-ISSN: 1471-9053 | DOI: https://dx.doi.org/10.1093/pcp/pci143
Homoglutathione Confers Tolerance to Acifluorfen in Transgenic Tobacco Plants Expressing Soybean Homoglutathione Synthetase

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Homoglutathione (hGSH), which is present in some leguminous plants, is preferred over GSH in in vitro conjugation of acifluorfen and fomesafen by glutathione S-transferase. To investigate the function of hGSH in in vivo detoxification of xenobiotics, we evaluated herbicide tolerance of transgenic tobacco plants expressing soybean homoglutathione synthetase in the cytosol or chloroplasts. Transgenic plants synthesizing hGSH in the cytosol were more tolerant to acifluorfen than wild-type plants, whereas enhanced tolerance to fomesafen was not observed. Transgenic plants synthesizing hGSH in the chloroplasts showed no enhanced tolerance to acifluorfen or fomesafen.

Keywords: Acifluorfen; Glutathione; Glutathione S-transferase; Herbicide tolerance; Homoglutathione; Transgenic tobacco; CDNB, 1-chloro-2,4-dinitrobenzene; GSHS, glutathione synthetase; GST, glutathione S-transferase; hGSH, homoglutathione; hGSHS, homoglutathione synthetase

Journal Article.  3223 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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