Journal Article

Dissection of the Phosphorylation of Rice DELLA Protein, SLENDER RICE1

Hironori Itoh, Akie Sasaki, Miyako Ueguchi-Tanaka, Kanako Ishiyama, Masatomo Kobayashi, Yasuko Hasegawa, Eiichi Minami, Motoyuki Ashikari and Makoto Matsuoka

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 46, issue 8, pages 1392-1399
Published in print August 2005 | ISSN: 0032-0781
Published online August 2005 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pci152
Dissection of the Phosphorylation of Rice DELLA Protein, SLENDER RICE1

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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DELLA proteins are repressors of gibberellin signaling in plants. Our previous studies have indicated that gibberellin signaling is derepressed by SCFGID2-mediated proteolysis of the DELLA protein, SLENDER RICE1 (SLR1), in rice. In addition, the gibberellin-dependent increase of phosphorylated SLR1 in the loss-of-function gid2 mutant suggests that the SCFGID2-mediated degradation of SLR1 might be initiated by gibberellin-dependent phosphorylation. To confirm the role of phosphorylation of SLR1 in its gibberellin-dependent degradation, we revealed that SLR1 is phosphorylated on an N-terminal serine residue(s) within the DELLA/TVHYNP and polyS/T/V domain. However, gibberellin-induced phosphorylation in these regions was not observed in the gid2 mutant following the constitutive expression of SLR1 under the control of the rice actin1 promoter. Treatment with gibberellin induced both the phosphorylated and non-phosphorylated forms of SLR1 with similar induction kinetics in gid2 mutant cells. Both the phosphorylated and non-phosphorylated SLR1 proteins were degraded by gibberellin treatment with a similar half-life in the rice callus cells, and both proteins interacted with recombinant glutathione S-transferase (GST)–GID2. These results demonstrate that the phosphorylation of SLR1 is independent of its degradation and is dispensable for the interaction of SLR1 with the GID2/F-box protein.

Keywords: DELLA protein; F-box protein; Gibberellin; Phosphorylation; Rice (Oryza sativa L.); CIP, calf intestine alkaline phosphatase; cps, CDP synthase; GFP, green fluorescent protein; gid2, gibberellin insensitive dwarf 2; GST, glutathione S-transferase; HA, hemagglutinin; WT, wild type

Journal Article.  5823 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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