Journal Article

A Sex Pheromone, Protoplast Release-inducing Protein (PR-IP) Inducer, Induces Sexual Cell Division and Production of PR-IP in <i>Closterium</i>

Yuki Tsuchikane, Tadashi Fujii, Motomi Ito and Hiroyuki Sekimoto

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 46, issue 9, pages 1472-1476
Published in print September 2005 | ISSN: 0032-0781
Published online September 2005 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pci158
A Sex Pheromone, Protoplast Release-inducing Protein (PR-IP) Inducer, Induces Sexual Cell Division and Production of PR-IP in Closterium

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The sex pheromone protoplast release-inducing protein (PR-IP) inducer and a sexual cell division-inducing pheromone-minus (SCD-IP-minus) that mediates the sexual reproduction of the heterothallic Closterium peracerosum-strigosum-littorale (C. psl) complex were investigated in this study. Recombinant PR-IP inducer produced by yeast cells was prepared and assayed for production of PR-IP and induction of SCD. Both biological activities were observed after treating mating-type plus (mt+) cells with the recombinant pheromone. SCD was induced by exposure to a lower concentration of the same pheromone and by a shorter treatment period with the pheromone than was production of PR-IP. This indicates that the previously characterized PR-IP inducer has both PR-IP-inducing and SCD-inducing activities with mt+ cells, although the inducing mechanisms of the two pheromones differ.

Keywords: Closterium; Conjugation; Pheromone; Sexual cell division; Sexual reproduction; C. psl, Closterium peracerosum-strigosum-littorale; MI medium, mating-inducing medium; mt+, mating-type plus; mt–, mating-type minus; PR-IP, protoplast release-inducing protein; SCD, sexual cell division; SCD-IP, sexual cell division-inducing pheromone

Journal Article.  2953 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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