Journal Article

The <i>IRREGULAR TRICHOME BRANCH</i> loci regulate trichome elongation in <i>Arabidopsis</i>

Xiaoguo Zhang, Paris H. Grey, Sujatha Krishnakumar and David G. Oppenheimer

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 46, issue 9, pages 1549-1560
Published in print September 2005 | ISSN: 0032-0781
Published online September 2005 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pci168
The IRREGULAR TRICHOME BRANCH loci regulate trichome elongation in Arabidopsis

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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The proper control of cell expansion is vital to plant development. It is responsible for shaping individual cells and, together with cell division, it plays a lead role in shaping plant organs. Much of the underlying mechanism by which plant cells expand anisotropically is not understood. We are taking a genetic approach to cell expansion by isolating mutants that affect the branching pattern of Arabidopsis trichomes. Here we report the identification of four new loci that control trichome morphogenesis. These loci were named the IRREGULAR TRICHOME BRANCH (ITB) loci because of the deleterious effects on branch position and length in the mutants. Our analysis of branch expansion in itb mutants shows that the ITB genes act as positive regulators of branch elongation, and that the branch position defects are caused by altered expansion of the trichome stalk. The itb mutations display synergistic effects in double mutant combinations with certain branch number mutations, suggesting that the ITB genes also play key roles in branch initiation. These results demonstrate that the ITB genes are key regulators of anisotropic cell expansion in trichomes.

Keywords: Arabidopsis thaliana; Cell shape; Morphogenesis; Trichome; EGTA, ethylene glycol-bis (β-aminoethyl ether)-N,N,N′,N′-tetraacetic acid; MT, microtubule; PBST, phosphate-buffered saline containing 0.05% Triton X-100; PIPES, 1,4-piperazine diethane sulfonic acid; SSLP, simple sequence length polymorphism

Journal Article.  7623 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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