Journal Article

A Suppressor Mutation in the α-Phycocyanin Gene in the Light/Glucose-sensitive Phenotype of the <i>psbK</i>-disruptant of the Cyanobacterium <i>Synechocystis</i> sp. PCC 6803

Mari Kobayashi, Katsuhiko Okada and Masahiko Ikeuchi

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 46, issue 9, pages 1561-1567
Published in print September 2005 | ISSN: 0032-0781
Published online September 2005 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pci169
A Suppressor Mutation in the α-Phycocyanin Gene in the Light/Glucose-sensitive Phenotype of the psbK-disruptant of the Cyanobacterium Synechocystis sp. PCC 6803

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psbK encodes a small transmembrane component of PSII. Here we report that the psbK-disruptant of Synechocystis sp. PCC 6803 cannot survive under photomixotrophic conditions of light and glucose after transient growth, while the wild type is able to grow. A spontaneous yellow-green mutant that recovered the sustained growth under the same conditions was isolated from the psbK-disruptant. Instead of recovery, the mutant largely lost photoautotrophic growth. By phenotype complementation, the mutation was identified in cpcA as a sequence replacement with a close downstream segment, generating an inverted repeat of 23 bp. The mutant phenotype was characterized by (i) the complete loss of α- and β-phycocyanin; (ii) increased accumulation of PSII; and (iii) greatly reduced transcripts harboring cpcA in abundance and in size. The inverted repeat generated in cpcA probably led to the early termination of transcription. A possible mechanism for such a mutation is discussed.

Keywords: cpcA; Light/glucose-sensitive; Phycocyanin; psbK; Sequence substitution; Template switching; ApcA, α-allophycocyanin; ApcB, β-allophycocyanin; ApcE, phycobilisome anchor protein; CpcA, α-phycocyanin; CpcB, β-phycocyanin

Journal Article.  5423 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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