Journal Article

Expression Profile of the Genes for Rice Cation/H<b><sup>+</sup></b> Exchanger Family and Functional Analysis in Yeast

Takehiro Kamiya, Taro Akahori and Masayoshi Maeshima

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 46, issue 10, pages 1735-1740
Published in print October 2005 | ISSN: 0032-0781
Published online October 2005 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pci173
Expression Profile of the Genes for Rice Cation/H+ Exchanger Family and Functional Analysis in Yeast

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We identified five cation/H+ exchangers (CAX) from rice, and phylogenetically divided them into two clusters. Gene expression and absolute amounts of mRNA in different organs were analyzed by real-time PCR. OsCAX1a showed high expression in almost all organs. OsCAX1b and OsCAX1c were detected in a limited number of organs and their expression levels were very low. The mRNA levels of OsCAX2 and OsCAX3 varied with the organ. OsCAXs were heterologously expressed in yeast to characterize the ion transport activity. All exchangers, except for OsCAX2, conferred tolerance to calcium. OsCAX1a and OsCAX3 conferred tolerance to manganese. The diversity of expression sites and substrates suggest the broad range function of CAX.

Keywords: Cation/H exchanger; Gene expression; Oryza sativa; Vacuole; Yeast; CAX, cation/H+ exchanger; RACE, rapid amplification of cDNA ends

Journal Article.  3247 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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