Journal Article

LIP19, a Basic Region Leucine Zipper Protein, is a Fos-like Molecular Switch in the Cold Signaling of Rice Plants

Hidekazu Shimizu, Kazuhito Sato, Thomas Berberich, Atsushi Miyazaki, Rei Ozaki, Ryozo Imai and Tomonobu Kusano

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 46, issue 10, pages 1623-1634
Published in print October 2005 | ISSN: 0032-0781
Published online October 2005 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pci178
LIP19, a Basic Region Leucine Zipper Protein, is a Fos-like Molecular Switch in the Cold Signaling of Rice Plants

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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The rice low-temperature-induced lip19 gene encodes a 148-amino-acid basic region/leucine zipper (bZIP) protein, termed LIP19. In this study we characterized LIP19 and showed that it lacks the usual ability of bZIP proteins to homodimerize and to bind DNA, as does the Fos protein in mammals. Using a yeast two-hybrid system, the cDNA clones whose products interact with LIP19 were screened. This search revealed a clone termed OsOBF1 (Oryza sativa OBF1) that encodes a new bZIP protein (OsOBF1). This protein forms a homodimer and binds to the hexamer motif sequence (5′-ACGTCA-3′). The protein–protein interaction in homo- and hetero-combinations between LIP19 and OsOBF1 was confirmed in vitro and in planta. LIP19 and OsOBF1 most likely interact with each other more strongly than OsOBF1 interacts with itself, and the resulting heterodimer binds to the C/G hybrid sequence but not to the hexamer sequence. Whereas the expression patterns of lip19 and OsOBF1 in response to low temperatures were totally opposite, the locations of their expression were almost identical. Based upon the presented data, we propose a model describing the low-temperature signal switching mediated by LIP19 in rice.

Keywords: bZIP protein; Cold signaling; DNA binding; Heterodimer; Molecular switch; Rice; BF, binding form; BiFC, bimolecular fluorescence complementation; bZIP, basic region/leucine zipper; DIG, digoxigenin; DTT, dithiothreitol; EMSA, electrophoretic mobility shift assay; GFP, green fluorescent protein; GST, glutathione S-transferase; IPTG, isopropyl-β-d-thiogalactopyranoside; OBF1, ocs-element Binding Factor 1; ocs, octopine synthase; ONPG, o-nitrophenyl-β-d-galactopyranoside; ORF, open reading frame; OsOBF1, Oryza sativa OBF1; PMSF, phenylmethylsulfonyl fluoride; SD, synthetic drop-out; TF, transcription factor; YFP, yellow fluorescent protein

Journal Article.  7654 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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