Journal Article

Localization of NAD-Isocitrate Dehydrogenase and Glutamate Dehydrogenase in Rice Roots: Candidates for Providing Carbon Skeletons to NADH-Glutamate Synthase

Tomomi Abiko, Mitsuhiro Obara, Akiko Ushioda, Toshihiko Hayakawa, Michael Hodges and Tomoyuki Yamaya

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 46, issue 10, pages 1724-1734
Published in print October 2005 | ISSN: 0032-0781
Published online October 2005 | e-ISSN: 1471-9053 | DOI: https://dx.doi.org/10.1093/pcp/pci188
Localization of NAD-Isocitrate Dehydrogenase and Glutamate Dehydrogenase in Rice Roots: Candidates for Providing Carbon Skeletons to NADH-Glutamate Synthase

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In rice roots, transient and cell-type-specific accumulation of both mRNA and protein for NADH-dependent glutamate synthase (NADH-GOGAT) occurs after the supply of NH4 + ions. In order to better understand the origin of 2-oxoglutarate for this reaction, we focused on mitochondrial NAD-dependent isocitrate dehydrogenase (IDH) and glutamate dehydrogenase (GDH) in rice roots. Six rice cDNAs encoding a single catalytic (OsIDHa) and two regulatory (OsIDHc;1, OsIDHc;2) IDH subunits and three GDH proteins (OsGDH1-3) were isolated. These genes, except OsGDH3, were expressed in the roots. Real-time PCR analysis showed that OsIDHa and OsIDHc;1 transcripts, but not OsGDH1 and OsGDH2 transcripts, accumulated in a similar manner to NADH-GOGAT mRNA along the crown roots after the supply of different forms of inorganic nitrogen. Furthermore, immunolocalization studies revealed the NH4 + induction of IDHa protein in two cell layers of the root surface, i.e. epidermis and exodermis, where NADH-GOGAT also accumulated. The possible relationship between NADH-GOGAT, IDH and GDH is discussed.

Keywords: Carbon and nitrogen metabolisms; Glutamate dehydrogenase; Isocitrate dehydrogenase; NADH-glutamate synthase; 2-Oxoglutarate; Rice; GDH, glutamate dehydrogenase; GOGAT, glutamate synthase; IDH, NAD-dependent isocitrate dehydrogenase; ICDH, NADP-dependent isocitrate dehydrogenase

Journal Article.  6047 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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