Journal Article

The Star-type Color Pattern in <i>Petunia hybrida</i> ‘Red Star’ Flowers is Induced by Sequence-Specific Degradation of <i>Chalcone Synthase</i> RNA

Maiko Koseki, Kazunori Goto, Chikara Masuta and Akira Kanazawa

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 46, issue 11, pages 1879-1883
Published in print November 2005 | ISSN: 0032-0781
Published online November 2005 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pci192
The Star-type Color Pattern in Petunia hybrida ‘Red Star’ Flowers is Induced by Sequence-Specific Degradation of Chalcone Synthase RNA

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  • Biochemistry
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Petunia hybrida ‘Red Star’ is a variety whose flowers exhibit a star-type red and white bicolor pattern. We analyzed the mRNA levels of six genes involved in anthocyanin biosynthesis. Only the level of chalcone synthase (CHS) mRNA was depressed in the unpigmented flower sectors. Both transcriptional activity and the accumulation of short interfering RNA of CHS in the unpigmented sectors were detected. Viral infection blocked the generation of CHS-silenced sectors. These results indicate that sequence-specific degradation of CHS RNA is the primary cause of the formation of white sectors in ‘Red Star’ flowers.

Keywords: Chalcone synthase; Cucumber mosaic virus; Petunia hybrida; Post-transcriptional gene silencing; RNA silencing; Short interfering RNA; 3GT, flavonoid 3-glucosyltransferase; ANS, anthocyanidin synthase; CHI, chalcone isomerase; CHS, chalcone synthase; CMV-O, cucumber mosaic virus O strain; DFR, dihydroflavonol 4-reductase; dsRNA, double-stranded RNA; F3H, flavanone 3-hydroxylase; miRNA, micro RNA; PTGS, post-transcriptional gene silencing; RISC, RNA-induced silencing complex; RNAi, RNA interference; RT-PCR, reverse transcriptase PCR; siRNA, short interfering RNA; TGS, transcriptional gene silencing

Journal Article.  3335 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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