Journal Article

Ionic Mechanism of Mechano-perception in Characeae

Kosei Iwabuchi, Toshiyuki Kaneko and Munehiro Kikuyama

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 46, issue 11, pages 1863-1871
Published in print November 2005 | ISSN: 0032-0781
Published online November 2005 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pci204
Ionic Mechanism of Mechano-perception in Characeae

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  • Biochemistry
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Characean internodal cells generate receptor potential in response to mechanical stimuli. We studied responses of internodal cells to a long-lasting stimulus and the results were as follows. (i) The cell generated receptor potential at the moment of both compression and decompression. (ii) The receptor potential (ΔE m) was significantly larger at the moment of decompression than at compression. (iii) The longer the duration of the stimulus, the larger was the magnitude of ΔE m at the moment of decompression. (iv) Aequorin studies revealed that the increase in [Ca2+]c (Δ[Ca2+]c) took place at the moment of both compression and decompression. (v) The amplitude of Δ[Ca2+]c was larger at the moment of decompression than at compression, as was the case for ΔE m. It was suggested that the amplitude of the receptor potential had a tight correlation with the degree of membrane deformation. We discussed the ionic mechanism of mechano-perception under a long-lasting stimulus in relation to mechanosensitive activation of Ca2+ channels at the plasma membrane.

Keywords: Ca2+; Characeae; Mechanosensitive ion channel; Membrane deformation; Membrane potential; Receptor potential; APW, artificial pond water; [Ca2+]c, cytoplasmic free calcium concentration; Em, membrane potential; ΔEm, receptor potential; (ΔEm)S, ΔEm at the start of stimulation; (ΔEm)E, ΔEm at the end of stimulation; (ΔEm)0.1, ΔEm on stimulation for 0.1 s; (ΔEm)L, (ΔEm)E on stimulation at L mm from the node; 55KCl-APW, APW containing 55 mM KCl; 100KCl+10CaCl2-APW, APW containing 100 mM KCl and 10 mM CaCl2; LED, light-emitting diode; PM, photomultiplier; 100sorbitol-APW, APW containing 100 mM sorbitol

Journal Article.  6169 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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