Journal Article

A Mitogen-activated Protein Kinase NtMPK4 Activated by SIPKK is Required for Jasmonic Acid Signaling and Involved in Ozone Tolerance via Stomatal Movement in Tobacco

Kenji Gomi, Daisuke Ogawa, Shinpei Katou, Hiroshi Kamada, Nobuyoshi Nakajima, Hikaru Saji, Takashi Soyano, Michiko Sasabe, Yasunori Machida, Ichiro Mitsuhara, Yuko Ohashi and Shigemi Seo

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 46, issue 12, pages 1902-1914
Published in print December 2005 | ISSN: 0032-0781
Published online December 2005 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pci211
A Mitogen-activated Protein Kinase NtMPK4 Activated by SIPKK is Required for Jasmonic Acid Signaling and Involved in Ozone Tolerance via Stomatal Movement in Tobacco

More Like This

Show all results sharing these subjects:

  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

GO

Show Summary Details

Preview

The mitogen-activated protein kinase (MAPK) cascade is involved in responses to biotic and abiotic stress in plants. In this study, we isolated a new MAPK, NtMPK4, which is a tobacco homolog of Arabidopsis MPK4 (AtMPK4). NtMPK4 was activated by wounding along with two other wound-responsive tobacco MAPKs, WIPK and SIPK. We found that NtMPK4 was activated by salicylic acid-induced protein kinase kinase (SIPKK), which has been isolated as an SIPK-interacting MAPK kinase. In NtMPK4 activity-suppressed tobacco, wound-induced expression of jasmonic acid (JA)-responsive genes was inhibited. NtMPK4-silenced plants showed enhanced sensitivity to ozone. Inversely, transgenic tobacco plants, in which SIPKK or the constitutively active type SIPKKEE was overexpressed, exhibited greater responsiveness to wounding with enhanced resistance to ozone. We further found that NtMPK4 was expressed preferentially in epidermis, and the enhanced sensitivity to ozone in NtMPK4-silenced plants was caused by an abnormal regulation of stomatal closure in an ABA-independent manner. These results suggest that NtMPK4 is involved in JA signaling and in stomatal movement.

Keywords: Jasmonic acid; MAP kinase; Ozone; Stomata; Wound response; ACS, 1-aminocyclopropane-1-carboxylate synthase; AOS, allene oxide synthase; GST, glutathione S-transferase; JA, jasmonic acid; MAPK/MPK, mitogen-activated protein kinase; MAPKK/MEK/MKK, MAPK kinase; MBP, myelin basic protein; ORF, open reading frame; PI-II, proteinase inhibitor-II; PPFD, photosynthetic photon flux density; PR, pathogenesis-related; RACE, rapid amplification of cDNA ends; RT–PCR, reverse transcription–PCR; SA, salicylic acid; SIPK, salicylic acid-induced protein kinase; SIPKK, salicylic acid-induced protein kinase kinase; WIPK, wound-induced protein kinase

Journal Article.  8350 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

Full text: subscription required

How to subscribe Recommend to my Librarian

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content.