Journal Article

Defense and Resistance-inducing Activities in Tobacco of the Sulfated β-1,3 glucan PS3 and its Synergistic Activities with the Unsulfated Molecule

Rozenn Ménard, Patrice de Ruffray, Bernard Fritig, Jean-Claude Yvin and Serge Kauffmann

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 46, issue 12, pages 1964-1972
Published in print December 2005 | ISSN: 0032-0781
Published online December 2005 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pci212
Defense and Resistance-inducing Activities in Tobacco of the Sulfated β-1,3 glucan PS3 and its Synergistic Activities with the Unsulfated Molecule

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Laminarin, a β-1,3 glucan with single β-glucose branches at position 6, was chemically sulfated to produce PS3 with a degree of sulfation of 2.4. PS3 has previously been shown to activate the salicylic acid (SA) signaling pathway in infiltrated tobacco and Arabidopsis thaliana leaf tissues. Here, we investigated whether PS3 induces systemic defense and resistance responses in tobacco. Using a radiolabeled compound, it was first demonstrated that PS3 remains strictly localized to the infiltrated tissues. PS3 is also resistant to β-glucanase degradation. In transgenic PR1–β-glucuronidase (GUS) tobacco plants, PS3 causes a strong increase in GUS activity in treated tissues but none in untreated leaves. PS3-infiltrated tissues challenged with tobacco mosaic virus (TMV) 8 d after elicitor application show a decrease in both the lesion number and the lesion size, whereas treatment with laminarin, the unsulfated native glucan, affected only the lesion number. PS3 does not induce systemic acquired resistance to TMV. PS3 and laminarin show synergistic effects in promoting the oxidative burst in tobacco cell suspensions and in increasing the expression of genes encoding O-methyltransferases of the phenylpropanoid pathway in tobacco plants. No synergistic effect was observed on the expression of either the SA-dependent acidic PR1 gene or the ethylene-dependent basic PR5 gene in tobacco plants.

Keywords: Defense; Glucan; Resistance; Synergy; Tobacco; ANOVA, analysis of variance; CCoAOMT, caffeoyl CoA-O-methyltransferase; F, fluorosceinamine; GUS, β-glucuronidase; HR, hypersensitive response; LAR, localized acquired resistance; OMT, O-methyltransferase; Pms, Phytophthora megasperma var. sojae; PR, pathogenesis related; RLU, relative light units; ROI, reactive oxygen species; RT–PCR, reverse transcription–PCR; SA, salicylic acid; SAR, systemic acquired resistance; TMV, tobacco mosaic virus

Journal Article.  7383 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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