Journal Article

Apoplastic Glycosidases Active Against Xyloglucan Oligosaccharides of <i>Arabidopsis thaliana</i>

Natalia Iglesias, José Antonio Abelenda, María Rodiño, Javier Sampedro, Gloria Revilla and Ignacio Zarra

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 47, issue 1, pages 55-63
Published in print January 2006 | ISSN: 0032-0781
Published online January 2006 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pci223
Apoplastic Glycosidases Active Against Xyloglucan Oligosaccharides of Arabidopsis thaliana

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All four glycanases necessary for the degradation of xyloglucan oligosaccharides (α-fucosidase, α-xylosidase, β-galactosidase and β-glucosidase) were found in the apoplastic fluid of Arabidopsis thaliana. These activities acted cooperatively on xyloglucan oligosaccharides (XLFG), leading to the sequential formation of XXFG, XXLG, XXXG, GXXG and XXG, as identified by matrix-assisted laser desorption ionization time of flight (MALDI-TOF). AtFXG1 (At1g67830) and AtXYL1 (At1g68560) had been previously identified as the Arabidopsis genes coding for α-fucosidase and α-xylosidase, respectively. As for the genes coding for β-galactosidase activity, we identified in phylogenetic trees 12 candidates from family 35 of glycoside hydrolases. Similarly, four genes from family 3 were selected as possible β-glucosidases active on xyloglucan. The expression level of all the selected genes was studied in different plant regions (young and mature rosette leaves, apical and basal region of the inflorescence stem, roots, flower and siliques) using quantitative real-time reverse transcription–PCR. The expression patterns were very diverse as well as their relationship with growth rates, showing a very complex situation. This could lead to highly varying proportions of the different xyloglucan oligosaccharides in different plant regions and developmental stages.

Keywords: Arabidopsis; α-Fucosidase; β-Galactosidase; β-Glucosidase; Xyloglucan; α-Xylosidase; MALDI-TOF, matrix-assisted laser desorption ionization time of flight; PSD, post-source decay; RT–PCR, reverse transcription–PCR

Journal Article.  5570 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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