Journal Article

Leaf Yellowing and Anthocyanin Accumulation are Two Genetically Independent Strategies in Response to Nitrogen Limitation in <i>Arabidopsis thaliana</i>

Céline Diaz, Vera Saliba-Colombani, Olivier Loudet, Pierre Belluomo, Laurence Moreau, Françoise Daniel-Vedele, Jean-François Morot-Gaudry and Céline Masclaux-Daubresse

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 47, issue 1, pages 74-83
Published in print January 2006 | ISSN: 0032-0781
Published online January 2006 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pci225
Leaf Yellowing and Anthocyanin Accumulation are Two Genetically Independent Strategies in Response to Nitrogen Limitation in Arabidopsis thaliana

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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For the first time in Arabidopsis thaliana, this work proposes the identification of quantitative trait loci (QTLs) associated with leaf senescence and stress response symptoms such as yellowing and anthocyanin-associated redness. When Arabidopsis plants were cultivated under low nitrogen conditions, we observed that both yellowing of the old leaves of the rosette and whole rosette redness were promoted. Leaf yellowing is a senescence symptom related to chlorophyll breakdown. Redness is a symptom of anthocyanin accumulation related to whole plant ageing and nutrient limitation. In this work, Arabidopsis is used as a model system to dissect the genetic variation of these parameters by QTL mapping in the 415 recombinant inbred lines of the Bay-0×Shahdara population. Fifteen new QTLs and two epistatic interactions were described in this study. The yellowing of the rosette, estimated by visual notation and image processing, was controlled by four and five QTLs, respectively. The visual estimation of redness allowed us to detect six QTLs among which the major one explained 33% of the total variation. Two main QTLs were confirmed in near-isogenic lines (heterogenous inbred family; HIF), thus confirming the relevance of the visual notation of these traits. Co-localizations between QTLs for leaf yellowing, redness and nitrogen use efficiency described in a previous publication indicate complex interconnected pathways involved in both nitrogen management and senescence- and stress-related processes. No co-localization between QTLs for leaf yellowing and redness has been found, suggesting that the two characters are genetically independent.

Keywords: Anthocyanin; Heterogeneous inbred family; Near-isogenic line; Nitrogen; QTL; Senescence; AA, amino acid content; CIM, composite interval mapping; DM, dry matter; HIF, heterogenous inbred family; LAR, leaf area rating; NIL, near-isogenic line; NP, nitrogen content; QTL, quantitative trait locus; RIL, recombinant inbred line; RV, red visual notation; SD, short day; YP, yellowing by image processing; YV, yellowing by visual notation

Journal Article.  7441 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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