Journal Article

Expression Profiling Identifies Genes Expressed Early During Lint Fibre Initiation in Cotton

Yingru Wu, Adriane C. Machado, Rosemary G. White, Danny J. Llewellyn and Elizabeth S. Dennis

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 47, issue 1, pages 107-127
Published in print January 2006 | ISSN: 0032-0781
Published online January 2006 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pci228
Expression Profiling Identifies Genes Expressed Early During Lint Fibre Initiation in Cotton

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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Cotton fibres are a subset of single epidermal cells that elongate from the seed coat to produce the long cellulose strands or lint used for spinning into yarn. To identify genes that might regulate lint fibre initiation, expression profiles of 0 days post-anthesis (dpa) whole ovules from six reduced fibre or fibreless mutants were compared with wild-type linted cotton using cDNA microarrays. Numerous clones were differentially expressed, but when only those genes that are normally expressed in the ovule outer integument (where fibres develop) were considered, just 13 different cDNA clones were down-regulated in some or all of the mutants. These included: a Myb transcription factor (GhMyb25) similar to the Antirrhinum Myb AmMIXTA, a putative homeodomain protein (related to Arabidopsis ATML1), a cyclin D gene, some previously identified fibre-expressed structural and metabolic genes, such as lipid transfer protein, α-expansin and sucrose synthase, as well as some unknown genes. Laser capture microdissection and reverse transcription–PCR were used to show that both the GhMyb25 and the homeodomain gene were predominantly ovule specific and were up-regulated on the day of anthesis in fibre initials relative to adjacent non-fibre ovule epidermal cells. Their spatial and temporal expression pattern therefore coincided with the time and location of fibre initiation. Constitutive overexpression of GhMyb25 in transgenic tobacco resulted in an increase in branched long-stalked leaf trichomes. The involvement of cell cycle genes prompted DNA content measurements that indicated that fibre initials, like leaf trichomes, undergo DNA endoreduplication. Cotton fibre initiation therefore has some parallels with leaf trichome development, although the detailed molecular mechanisms are clearly different.

Keywords: Cotton; Expression profiling; Fibre development; Gossypium hirsutum; Microarray; Trichome; DAPI, 4′,6-diamidino-2-phenylindole; dpa, days post-anthesis; DP16, cultivar Deltapine 16; EST, expressed sequence tag; GL1, Glabrous 1; GL2, Glabrous 2; GUS, β-glucuronidase; LCM, laser capture microdissection; LTP, lipid transfer protein; SEM, scanning electron microscopy

Journal Article.  13874 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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