Journal Article

Accumulation of the Two Transcripts of the <i>N</i> gene, Conferring Resistance to <i>Tobacco Mosaic Virus</i>, is Probably Important for <i>N</i> Gene-dependent Hypersensitive Cell Death

Reona Takabatake, Shigemi Seo, Ichiro Mitsuhara, Shinnya Tsuda and Yuko Ohashi

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 47, issue 2, pages 254-261
Published in print February 2006 | ISSN: 0032-0781
Published online February 2006 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pci243
Accumulation of the Two Transcripts of the N gene, Conferring Resistance to Tobacco Mosaic Virus, is Probably Important for N Gene-dependent Hypersensitive Cell Death

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The N gene is a Toll/interleukin-1 receptor (TIR)–nucleotide-binding site (NBS)–leucine-rich repeat (LRR)-type resistance (R) gene that generates two alternative transcripts, N S and N L. N S encodes the full-length N protein while N L is predicted to encode a truncated form of the protein lacking most of the LRR region. We found that the two transcripts were accumulated at 20°C, a permissive temperature, but not at 30°C, a non-permissive temperature for the N gene, in tobacco mosaic virus (TMV)-inoculated leaves. When N gene-dependent cell death was triggered by transient 20°C treatment for 2–6 h, considerable levels of the transcripts were accumulated just before cell death, although the levels of N S were always higher. The accumulation was induced by transient expression of the 50 kDa helicase domain (p50) of TMV replicase which is the Avr component of N, but not by transient expression of NtMEK2 DD-mediated cell death or N gene-independent hypersensitive cell death. These results suggest that the accumulation of N S and N L is associated with the function of N and, above a certain threshold, triggers N-mediated hypersensitive cell death.

Keywords: Alternative splicing; Hypersensitive cell death; N resistance gene; Temperature sensitive; Tobacco mosaic virus; ACC, 1-amino-cyclopropane-1-carboxylic acid; CC, coiled coil; HR, hypersensitive reaction or response; Hsp, heat shock protein; JA, jasmonic acid; LRR, leucine-rich repeat; MAPK, mitogen-activated protein kinase; MeJA, methyl jasmonate; NBS, nucleotide-binding site; PR, pathogenesis-related; RT–PCR, reverse transcription–PCR; SA, salicylic acid; SIPK, SA-induced protein kinase; TIR, toll/interleukin-1 receptor; TMV, tobacco mosaic virus; WIPK, wound-induced protein kinase

Journal Article.  5177 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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