Journal Article

Cytosolic Dehydroascorbate Reductase is Important for Ozone Tolerance in <i>Arabidopsis</i> <i>thaliana</i>

Seiji Yoshida, Masanori Tamaoki, Takeshi Shikano, Nobuyoshi Nakajima, Daisuke Ogawa, Motohide Ioki, Mitsuko Aono, Akihiro Kubo, Hiroshi Kamada, Yasunori Inoue and Hikaru Saji

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 47, issue 2, pages 304-308
Published in print February 2006 | ISSN: 0032-0781
Published online February 2006 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pci246
Cytosolic Dehydroascorbate Reductase is Important for Ozone Tolerance in Arabidopsis thaliana

More Like This

Show all results sharing these subjects:

  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

GO

Show Summary Details

Preview

Dehydroascorbate reductase (DHAR) is a key component of the ascorbate recycling system. Three functional DHAR genes are encoded in the Arabidopsis genome. Ozone exposure increased the expression of the cytosolic DHAR (cytDHAR) gene alone. We characterized an Arabidopsis mutant with a deficient cytDHAR. The mutant completely lacked cytDHAR activity and was highly ozone sensitive. The amounts of total ascorbate and glutathione were similar in both lines, but the amount of apoplastic ascorbate in the mutant was 61.5% lower. These results indicate that the apoplastic ascorbate, which is generated through the reduction of DHA by cytDHAR, is important for ozone tolerance.

Keywords: Arabidopsis thaliana— Apoplast —Ascorbate; Dehydroascorbate reductase; Oxidative stress; Ozone.; AsA, ascorbate; DHA, dehydroascorbate; DHAR, dehydroascorbate reductase; ET, ethylene; PPFD, photosynthetic photon flux density; ROS, reactive oxygen species; RT–PCR, reverse transcription–PCR.

Journal Article.  3917 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

Full text: subscription required

How to subscribe Recommend to my Librarian

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content.