Journal Article

A Highly Efficient Transformation Protocol for Micro-Tom, a Model Cultivar for Tomato Functional Genomics

Hyeon-Jin Sun, Sayaka Uchii, Shin Watanabe and Hiroshi Ezura

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 47, issue 3, pages 426-431
Published in print March 2006 | ISSN: 0032-0781
Published online March 2006 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pci251
A Highly Efficient Transformation Protocol for Micro-Tom, a Model Cultivar for Tomato Functional Genomics

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We report a highly efficient protocol for the Agrobacterium-mediated genetic transformation of a miniature dwarf tomato (Lycopersicon esculentum), Micro-Tom, a model cultivar for tomato functional genomics. Cotyledon explants of tomato inoculated with Agrobacterium tumefaciens (Rhizobium radiobacter) C58C1RifR harboring the binary vector pIG121Hm generated a mass of chimeric non-transgenic and transgenic adventitious buds. Repeated shoot elongation from the mass of adventitious buds on selection media resulted in the production of multiple transgenic plants that originated from independent transformation events. The transformation efficiency exceeded 40% of the explants. This protocol could become a powerful tool for functional genomics in tomato.

Keywords: Agrobacterium tumefaciens (Rhizobium radiobacter); Functional genomics; Lycopersicon esculentum L.; Micro-Tom; Transformation.; AS, acetosyringone; EMS, ethyl methanesulfonate; EST, expressed sequence tag; GUS, β-glucuronidase; MS, Murashige and Skoog.

Journal Article.  4064 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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