Journal Article

Ozone-induced Expression of the Arabidopsis <i>FAD7</i> Gene Requires Salicylic Acid, but not <i>NPR1</i> and <i>SID2</i>

Takashi Yaeno, Bunnai Saito, Tsutomu Katsuki and Koh Iba

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 47, issue 3, pages 355-362
Published in print March 2006 | ISSN: 0032-0781
Published online March 2006 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pci253
Ozone-induced Expression of the Arabidopsis FAD7 Gene Requires Salicylic Acid, but not NPR1 and SID2

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The Arabidopsis FAD7 gene encodes a plastid ω-3 fatty acid desaturase that catalyzes the desaturation of dienoic fatty acids to trienoic fatty acids in chloroplast membrane lipids. The expression of FAD7 was rapidly and locally induced by ozone exposure, which causes oxidative responses equivalent to pathogen-induced hypersensitive responses and subsequently activates various defense-related genes. This induction was reduced in salicylic acid (SA)-deficient NahG plants expressing SA hydroxylase, but was unaffected in etr1 and jar1 mutants, which are insensitive to ethylene and jasmonic acid (JA), respectively. The SA dependence of the FAD7 induction was confirmed by the exogenous application of SA. SA-induced expression of FAD7 in the npr1 mutant which is defective in an SA signaling pathway occurred to the same extent as in the wild type. Furthermore, in the sid2 mutant which lacks an enzyme required for SA biosynthesis, the expression of FAD7 was induced by ozone exposure. These results suggest that the ozone-induced expression of FAD7 gene requires SA, but not ethylene, JA, NPR1 and SID2.

Keywords: FAD7; Fatty acid desaturase; NPR1; Salicylic acid; SID2; AIP, 2-aminoindan-2-phosphonic acid; BTH, benzo(1,2,3)thiadiazole-7-carbothoic acid; FAD, fatty acid desaturase; GUS, β-glucuronidase; HR, hypersensitive response; ICS, isochorismate synthase; JA, jasmonic acid; NBT, nitroblue tetrazolium; PAL, phenylalanine ammonia lyase; PR, pathogenesis-related; SA, salicylic acid; SAR, systemic acquired resistance; TA, trienoic fatty acid

Journal Article.  5942 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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