Journal Article

Loss of AtPDR8, a Plasma Membrane ABC Transporter of <i>Arabidopsis thaliana</i>, Causes Hypersensitive Cell Death Upon Pathogen Infection

Yoshihiro Kobae, Tetsuro Sekino, Hirofumi Yoshioka, Tsuyoshi Nakagawa, Enrico Martinoia and Masayoshi Maeshima

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 47, issue 3, pages 309-318
Published in print March 2006 | ISSN: 0032-0781
Published online March 2006 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcj001
Loss of AtPDR8, a Plasma Membrane ABC Transporter of Arabidopsis thaliana, Causes Hypersensitive Cell Death Upon Pathogen Infection

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Plants contain a large number of ATP-binding cassette (ABC) transporters belonging to different subclasses. AtPDR8 is the only member of the pleiotropic drug resistance (PDR) ABC transporter subclass in Arabidopsis that is constitutively highly expressed. In transgenic Arabidopsis plants harboring the AtPDR8 promoter fused to β-glucuronidase (GUS), reporter expression was shown to be strong in the stomata and hydathode. In the stomata, transcripts of AtPDR8 were particularly frequent in the cells surrounding air spaces. Subcellular fractionation and immunochemical analysis showed that AtPDR8 was localized in the plasma membrane. When a knockout mutant of AtPDR8 (atpdr8) was infected with bacterial and oomycete pathogens, the plants exhibited chlorotic lesions and a hypersensitive response (HR)-like cell death. Cell death was detected in the atpdr8 mutants within 10 h of infection with the virulent bacterial pathogen, Pseudomonas syringae. As a result, the growth of P. syringae in the leaves of the atpdr8 mutant was reduced to 1% of that in the wild type. The defense response genes, PR-1, PR-2, PR-5, VPEγ, AtrbohD and AtrbohF were highly expressed when the mutant plants were grown under non-sterile conditions. The expression of the AtPDR8 gene was enhanced by infection of virulent and avirulent bacterial pathogens. Our results indicate that AtPDR8 is a key factor controlling the extent of cell death in the defense response and suggest that AtPDR8 transports some substance(s) which is closely related to the response of plants to pathogens.

Keywords: ABC transporter; Arabidopsis; Non-host resistance; PDR; ABC, ATP-binding cassette; AHA, plasma membrane H+-ATPase; DAB, 3,3′-diaminobenzidine; d.p.i., days post-inoculation; GUS, β-glucuronidase; HR, hypersensitive response; MDR, multidrug-resistant protein; MRP, multidrug resistance-associated protein; NBD, nucleotide-binding domain; PDR, pleiotropic drug resistance; PR, pathogenesis related; RT–PCR, reverse transcription–PCR; SAR, systemic aquired resistance; TMD, transmembrane domain; VHA-a, subunit a of the vacuolar H+-ATPase; VPE, vacuolar processing enzyme; X-Gluc, 5-bromo-4-chloro-3-indolyl-β-D-glucuronide

Journal Article.  6955 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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