Journal Article

Action Spectrum for Expression of the High Intensity Light-inducible <i>Lhc</i>-like Gene <i>Lhl4</i> in the Green Alga <i>Chlamydomonas reinhardtii</i>

Haruhiko Teramoto, Asako Ishii, Yukihiro Kimura, Koji Hasegawa, Shigeaki Nakazawa, Takanori Nakamura, Sho-ichi Higashi, Masakatsu Watanabe and Taka-aki Ono

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 47, issue 3, pages 419-425
Published in print March 2006 | ISSN: 0032-0781
Published online March 2006 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcj009
Action Spectrum for Expression of the High Intensity Light-inducible Lhc-like Gene Lhl4 in the Green Alga Chlamydomonas reinhardtii

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Lhl4 encodes a distant relative of light-harvesting Chl-a/b proteins in the green alga Chlamydomonas reinhardtii. Lhl4 mRNA markedly accumulated within 30 min after illumination and in proportion to the light intensity up to a fluence rate much higher than that required for photosynthesis. The high intensity light (HL)-induced accumulation of Lhl4 mRNA required continuous illumination, and the mRNA level rapidly decreased when the cells were placed in the dark. HL only slightly stabilized the mRNA, suggesting that the HL-induced expression of the Lhl4 gene is primarily regulated at the level of transcription. Blue light was more effective for inducing Lhl4 gene expression than green or red light, and far-red light had no effect. The action spectrum for Lhl4 gene expression was examined at wavelengths between 325 and 775 nm using the Okazaki Large Spectrograph. The obtained spectrum showed a distinct peak in the blue region (450 nm) and a shoulder in the UV-A region (375 nm). The curve in the spectrum rose steeply in the short wavelength UV region. In addition, we observed two minor peaks in the green (575 nm) and the red (675 nm) regions. The action spectrum suggests that a blue/UV-A light photoreceptor with a flavin-based chromophore participates in the HL response of Lhl4 gene expression. However, the hypersensitivity to near UV-B light suggests the involvement of an unidentified UV light perception system in the expression of the Lhl4 gene.

Keywords: Action spectrum; Blue/UV light photoreceptor; Chlamydomonas reinhardtii; Gene expression; High light response; Lhc supergene family; DBMIB, 2,5-dibromo-3-methyl-6-isopropyl-p-benzoquinone; ELIP, early light-inducible protein; HL, high intensity light; HLIP, high light-inducible protein; LED, light-emitting diode; LHC, light-harvesting chlorophyll-a/b-binding; LL, low intensity light; RT–PCR, reverse transcription–PCR

Journal Article.  5289 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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