Journal Article

An Alloplasmic Male-sterile Line of <i>Brassica oleracea</i> Harboring the Mitochondria from <i>Diplotaxis muralis</i> Expresses a Novel Chimeric Open Reading Frame, <i>orf72</i>

Tomotaka Shinada, Yosuke Kikuchi, Ryo Fujimoto and Sachie Kishitani

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 47, issue 4, pages 549-553
Published in print April 2006 | ISSN: 0032-0781
Published online April 2006 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcj014
An Alloplasmic Male-sterile Line of Brassica oleracea Harboring the Mitochondria from Diplotaxis muralis Expresses a Novel Chimeric Open Reading Frame, orf72

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Nuclear so-called fertility-restorer genes reverse the pollen sterility of cytoplasmic male-sterile (CMS) plants caused by disturbed mitochondrial–nuclear interactions. We identified a CMS-associated chimeric mitochondrial gene in an alloplasmic CMS line of Brassica oleracea in the ‘mur’ system. This novel chimeric gene, orf72, was found in the mitochondrial genome of donor cytoplasm. It was located downstream of normal rps7 and contained part of atp9 (atp9-b). It was expressed specifically on the nuclear background of CMS B. oleracea, partially suppressed in the fertility-restored line and entirely suppressed in the cytoplasmic donor.

Keywords: Alloplasm; Brassica oleracea; Cytoplasmic male sterility; Diplotaxis muralis; Mitochondrial gene; orf72; CMS, cytoplasmic male sterility; ORF, open reading frame

Journal Article.  2665 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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