Journal Article

Studies on Transcriptional Regulation of Endogenous Genes by ERF2 Transcription Factor in Tobacco Cells

Toshitsugu Nakano, Takumi Nishiuchi, Kaoru Suzuki, Tatsuhito Fujimura and Hideaki Shinshi

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 47, issue 4, pages 554-558
Published in print April 2006 | ISSN: 0032-0781
Published online April 2006 | e-ISSN: 1471-9053 | DOI: https://dx.doi.org/10.1093/pcp/pcj017
Studies on Transcriptional Regulation of Endogenous Genes by ERF2 Transcription Factor in Tobacco Cells

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  • Biochemistry
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In this study, we showed that overexpression of ethylene-responsive transcription factor (ERF) 2 activated the expression of endogenous genes that have the GCC box in their promoter region, in tobacco plants. These include not only a defense-related gene, CHN50, encoding class I basic chitinase, but also a transcriptional repressor gene, ERF3. In tobacco plants constitutively expressing ERF2:glucocorticoid receptor fusion protein, treatment with dexamethazone induced a rapid increase of ERF3 mRNA and a slow increase of CHN50 mRNA. These results suggest that an antagonistic interplay of ERF2 and ERF3 is involved in the transcriptional regulation of the class I basic chitinase genes in tobacco.

Keywords: Chitinase; ERF2; ERF3; GCC box; Tobacco; Transcription; ACC, 1-aminocyclopropane-1-carboxylic acid; AP2, Apetala2; CaMV35S, cauliflower mosaic virus 35S; DEX, dexamethasone; ERF, ethylene-responsive transcription factor; GR, glucocorticoid receptor; GUS, β-glucuronidase; LUC, luciferase; ORF, open reading frame; STS, silver thiosulfate

Journal Article.  2941 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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