Journal Article

Change in the Actin Cytoskeleton during Seismonastic Movement of <i>Mimosa pudica</i>

Nobuyuki Kanzawa, Yoshinori Hoshino, Makiko Chiba, Daisuke Hoshino, Hidetaka Kobayashi, Naomi Kamasawa, Yoshiro Kishi, Masako Osumi, Masazumi Sameshima and Takahide Tsuchiya

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 47, issue 4, pages 531-539
Published in print April 2006 | ISSN: 0032-0781
Published online April 2006 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcj022
Change in the Actin Cytoskeleton during Seismonastic Movement of Mimosa pudica

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The seismonastic movement of Mimosa pudica is triggered by a sudden loss of turgor pressure. In the present study, we compared the cell cytoskeleton by immunofluorescence analysis before and after movement, and the effects of actin- and microtubule-targeted drugs were examined by injecting them into the cut pulvinus. We found that fragmentation of actin filaments and microtubules occurs during bending, although the actin cytoskeleton, but not the microtubules, was involved in regulation of the movement. Transmission electron microscopy revealed that actin cables became loose after the bending. We injected phosphatase inhibitors into the severed pulvinus to examine the effects of such inhibitors on the actin cytoskeleton. We found that changes in actin isoforms, fragmentation of actin filaments and the bending movement were all inhibited after injection of a tyrosine phosphatase inhibitor. We thus propose that the phosphorylation status of actin at tyrosine residues affects the dynamic reorganization of actin filaments and causes seismonastic movement.

Keywords: Actin; Dephosphorylation; Microtubule; Mimosa pudica; Seismonastic movement; Tyrosine; BSA, bovine serum albumin; CB, cytochalasin B; CD, cytochalasin D; CS, cold stimulus; DMSO, dimethylsulfoxide; 2D-PAGE, two-dimensional PAGE; PAO, phenylarsine oxide; PBS, phosphate-buffered saline; SB, stabilizing buffer; TBS, Tris-buffered saline; TEM, transmission electron microscopy; TPBS, 0.05% Tween-20 in PBS

Journal Article.  6266 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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