Journal Article

Plant Lanosterol Synthase: Divergence of the Sterol and Triterpene Biosynthetic Pathways in Eukaryotes

Satoru Sawai, Tomoyoshi Akashi, Nozomu Sakurai, Hideyuki Suzuki, Daisuke Shibata, Shin-ichi Ayabe and Toshio Aoki

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 47, issue 5, pages 673-677
Published in print May 2006 | ISSN: 0032-0781
Published online May 2006 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcj032
Plant Lanosterol Synthase: Divergence of the Sterol and Triterpene Biosynthetic Pathways in Eukaryotes

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Sterols, essential eukaryotic constituents, are biosynthesized through either cyclic triterpenes, lanosterol (fungi and animals) or cycloartenol (plants). The cDNA for OSC7 of Lotus japonicus was shown to encode lanosterol synthase (LAS) by the complementation of a LAS-deficient mutant yeast and structural identification of the accumulated lanosterol. A double site-directed mutant of OSC7, in which amino acid residues crucial for the reaction specificity were changed to the cycloartenol synthase (CAS) type, produced parkeol and cycloartenol. The multiple amino acid sequence alignment of a conserved region suggests that the LAS of different eukaryotic lineages emerged from the ancestral CAS by convergent evolution.

Keywords: Cycloartenol; Lanosterol; Lotus japonicus; Oxidosqualene cyclase; BAS, β-amyrin synthase; CAS, cycloartenol synthase; EI-MS, electron ionization-mass spectrometry; GC–MS, gas chromatography–mass spectrometry; LAS, lanosterol synthase; LUP, lupeol synthase; NMR, nuclear magnetic resonance; ORF, open reading frame; OSC, oxidosqualene cyclase; TLC, thin-layer chromatography

Journal Article.  2783 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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