Journal Article

<i>Arabidopsis</i> UPF1 RNA Helicase for Nonsense-mediated mRNA Decay is Involved in Seed Size Control and is Essential for Growth

Masato Yoine, Terumi Nishii and Kenzo Nakamura

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 47, issue 5, pages 572-580
Published in print May 2006 | ISSN: 0032-0781
Published online May 2006 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcj035
Arabidopsis UPF1 RNA Helicase for Nonsense-mediated mRNA Decay is Involved in Seed Size Control and is Essential for Growth

More Like This

Show all results sharing these subjects:

  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

GO

Show Summary Details

Preview

UPF1 RNA helicase plays a central role in nonsense-mediated mRNA decay (NMD), which specifically recognizes aberrant mRNAs containing premature termination codons and targets them for degradation. Although NMD factors are highly conserved among eukaryotes, little is known about the role of NMD in plant growth and development. The lba1 mutant of Arabidopsis thaliana with a Gly851→Glu missense mutation in AtUPF1 yielded seeds that were on average 22% longer in the long axis and 35% heavier than the wild-type Col seeds. Expression of the wild-type AtUPF1 in this mutant reduced the seeds to a normal size. During early stages of seed development, globular to torpedo stages of the embryos were contained within seed sacs that were larger in lba1 than in Col. Furthermore, the distance between seeds in siliques was greater in lba1 than in Col, suggesting that the lba1 mutation may affect ovule development. Self-pollinated atupf1-3(+/–) plants heterozygous for AtUPF1 disrupted by T-DNA insertion developed atupf1-3(–/–) seeds with a size and shape similar to those of Col seeds. However, the atupf1-3(–/–) seedlings stopped growing after radicle emergence from the seed coat, and this seedling lethality was rescued by expressing the wild-type AtUPF1. These results suggest that the lba1 mutation in AtUPF1 maternally affects seed development and that AtUPF1 is essential for seedling growth.

Keywords: Arabidopsis thaliana; lba1/atupf1-1 mutant; Nonsense-mediated mRNA decay; Seed sac; Seed size; UPF1; dCAPS, derived cleaved amplified polymorphic sequence; NMD, nonsense-mediated mRNA decay; PTC, premature termination codon; RT–PCR, reverse transcription–PCR; UTR, untranslated region

Journal Article.  5862 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

Full text: subscription required

How to subscribe Recommend to my Librarian

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content.