Journal Article

Expressed Sequence Tag Analysis of <i>Lilium longiflorum</i> Generative Cells

Takashi Okada, Prem L. Bhalla and Mohan B. Singh

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 47, issue 6, pages 698-705
Published in print June 2006 | ISSN: 0032-0781
Published online June 2006 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcj040
Expressed Sequence Tag Analysis of Lilium longiflorum Generative Cells

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The generative cell, the male gametic cell progenitor in flowering plants, undergoes mitotic division to produce two sperm cells. We have examined the gene expression profile of the Lilium longiflorum (lily) generative cell by sequencing expressed sequence tags (ESTs). A total of 886 ESTs derived from the generative cell cDNA library were clustered into 637 unique ESTs comprising 123 cluster ESTs and 514 singleton ESTs. Thirty-nine percent of non-redundant ESTs showing similarity to Arabidopsis genes with known function were thus assigned putative functions. Genes related to the ubiquitin system were abundant, suggesting the key role of ubiquitin-dependent proteolysis in gametogenesis. A total of 168 and 129 non-redundant lily generative cell ESTs showed significant similarity to maize sperm cell ESTs and Arabidopsis male gametophyte-specific transcripts, respectively. Fifty-five ESTs appeared to have significant similarities to both maize sperm cell ESTs and Arabidopsis male gametophyte-specific genes, indicating conservation of male gamete-expressed genes across different plant genera. Thus our data provide a handle to identify Arabidopsis gamete-expressed genes and to investigate their function. Several of these genes are potential candidates for analyzing the molecular basis of fertilization and for investigating mechanisms of gamete-specific transcriptional regulation in Arabidopsis through bioinformatics-based approaches.

Keywords: Expressed sequence tag; Lilium longiflorum; Generative cell; Male gamete; Arabidopsis; Fertilization; DAPI, 4′,6-diamidino-2-phenylindole; E-value, expectation value; EST, expressed sequence tag; RT–PCR, reverse transcription–PCR

Journal Article.  5522 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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