Journal Article

Expression Profiling of Auxin-treated <i>Arabidopsis</i> Roots: Toward a Molecular Analysis of Lateral Root Emergence

Marta Laskowski, Steven Biller, Ken Stanley, Tymoteusz Kajstura and Reeta Prusty

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 47, issue 6, pages 788-792
Published in print June 2006 | ISSN: 0032-0781
Published online June 2006 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcj043
Expression Profiling of Auxin-treated Arabidopsis Roots: Toward a Molecular Analysis of Lateral Root Emergence

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Treating Arabidopsis roots with exogenous auxin results in dramatic changes in cellular processes including de novo induction of lateral roots which later emerge through the overlying cells. Microarray experiments reveal approximately 80 genes that are substantially up-regulated in the root over the first 12 h following auxin treatment. We hypothesize that the observed increase in expression of pectate lyase family genes leads to degradation of the pectin-rich middle lamellae, allowing cells in the parent root to separate cleanly. Differences in the degree of pectin methylation in lateral and parent roots may explain why lateral roots are not degraded themselves.

Keywords: Arabidopsis thaliana; Auxin; Cell separation; Lateral root; Microarray; Pectate lyase; EST, expressed sequence tag; PG, polygalacturonase; PL, pectate lyase; PME, pectin methyl esterase; qPCR, quantitative real-time PCR; SEM, scanning electron microscopy

Journal Article.  3610 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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