Journal Article

Increase of Homologous Recombination Frequency in Vascular Tissue of Arabidopsis Plants Exposed to Salt Stress

Alex Boyko, Darryl Hudson, Prasanna Bhomkar, Palak Kathiria and Igor Kovalchuk

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 47, issue 6, pages 736-742
Published in print June 2006 | ISSN: 0032-0781
Published online June 2006 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcj045
Increase of Homologous Recombination Frequency in Vascular Tissue of Arabidopsis Plants Exposed to Salt Stress

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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Here we analyzed the influence of salt stress on plant genome stability. Homologous recombination events were detected in transgenic Arabidopsis plants that carried in their genome a β-glucuronidase recombination marker. Recombination events were scored as blue sectors using a stereo microscope. Exposure to 50 mM salt resulted in a 3.0-fold increase in recombination frequency. To analyze the organ and tissue specificity of recombination events, we examined cross-sections of leaves, stems and roots. We found that nearly 30% of recombination events in plants grown under normal conditions and nearly 50% of events in plants grown on salt were undetected by the conventional method. Most of the recombination events represented a cluster/group of cells (12 on average), although events with single cells were also detected. Recombination events were very frequent in leaf mesophyll cells. On average, individual recombination events located on leaves contained more cells than events located on roots or stems. Analysis of recombination events in cross-sectioned tissue of salt-treated plants revealed a shift in the distribution of recombination events towards the vascular tissue. We discuss the significance of the finding for plant stress physiology.

Keywords: Arabidopsis thaliana; Homologous recombination; Salt stress; Tissue specific; Vascular tissue; DSB, double strand break; GUS, β-glucuronidase; HR, homologous recombination; MS, Murashige and Skoog; PBS, phosphate-buffered saline; X-glu, 5-bromo-4-chloro-3-indolyl glucuronide

Journal Article.  3883 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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