Journal Article

The Role of a Zinc Finger-containing Glycine-rich RNA-binding Protein During the Cold Adaptation Process in <i>Arabidopsis thaliana</i>

Yeon-Ok Kim and Hunseung Kang

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 47, issue 6, pages 793-798
Published in print June 2006 | ISSN: 0032-0781
Published online June 2006 | e-ISSN: 1471-9053 | DOI: https://dx.doi.org/10.1093/pcp/pcj047
The Role of a Zinc Finger-containing Glycine-rich RNA-binding Protein During the Cold Adaptation Process in Arabidopsis thaliana

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  • Biochemistry
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  • Plant Sciences and Forestry

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The mechanistic role of a glycine-rich RNA-binding protein designated atRZ-1a that contributes to enhance cold tolerance in Arabidopsis was investigated. Overexpression of atRZ-1a did not affect the expression of various cold-responsive genes such as COR6.6, COR15a, COR47, RD29A, RD29B and LTI29. Proteome analyses revealed that overexpression of atRZ-1a modulated the expression of several stress-responsive genes, and the transcript levels and RNA stability of these target genes were not affected by atRZ-1a. atRZ-1a successfully complements the cold sensitivity of Escherichia coli lacking four cold shock proteins. These results strongly suggest that atRZ-1a plays a role as an RNA chaperone during the cold adaptation process.

Keywords: Arabidopsis; Cold adaptation; Cold shock protein; Glycine-rich RNA-binding protein; RNA chaperone; CSD, cold shock domain; CSP, cold shock protein; GRP, glycine-rich RNA-binding protein; IPTG, isopropyl-β-d-thiogalactopyranoside; LB, Luria–Bertani; PEAMT, phosphoethanolamine N-methyltransferase; RBP, RNA-binding protein; RRM, RNA recognition motif; RT–PCR, reverse transcrition–PCR

Journal Article.  3762 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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