Journal Article

A Mutation in At-nMat1a, Which Encodes a Nuclear Gene Having High Similarity to Group II Intron Maturase, Causes Impaired Splicing of Mitochondrial NAD4 Transcript and Altered Carbon Metabolism in <i>Arabidopsis</i> <i>thaliana</i>

Naoki Nakagawa and Naoki Sakurai

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 47, issue 6, pages 772-783
Published in print June 2006 | ISSN: 0032-0781
Published online June 2006 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcj051
A Mutation in At-nMat1a, Which Encodes a Nuclear Gene Having High Similarity to Group II Intron Maturase, Causes Impaired Splicing of Mitochondrial NAD4 Transcript and Altered Carbon Metabolism in Arabidopsis thaliana

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To elucidate the mechanism of cellulose synthesis, we isolated a mutant of Arabidopsis (changed sensitivity to cellulose synthesis inhibitors 1, css1) that showed changed sensitivity to cellulose biosynthesis inhibitor. The analysis of phenotypes indicated that the css1 mutation influenced various fundamental metabolic pathways including amino acid metabolism, triacylglycerol degradation and polysaccharide synthesis (cellulose and starch) during the early stage of plant growth. Unexpectedly, the map-based cloning of the gene responsible for the css1 mutation identified a protein (At-nMat1a) that was assumed to be a splicing factor of the mitochondrial group II intron. In accordance with this result, this mutant exhibited improper splicing of the mitochondrial NAD4 transcript. We noticed that the phenotypes of the css1 mutant are similar to the responses to anoxia that hinders mitochondrial aerobic respiration. It seems that the defect in the function of mitochondria influences various aspects of fundamental cellular metabolism including cellulose synthesis. Our results suggested that sucrose synthase (SuSy), an enzyme involved in the biosynthesis of cellulose, plays key roles in the connection between mitochondria and cellulose synthesis. The isolation of the css1 mutant also provides a useful resource in the study of post-transcriptional gene regulation in mitochondria.

Keywords: Arabidopsis; Cellulose; 2,6-Dichlorobenzonitrile; Group II intron; Mitochondria; Sucrose synthase (SuSy); ADPG, ADP-glucose; BAC, bacterial artificial chromosome; COX2, cytochrome oxidase subunit 2; css1, changed sensitivity to cellulose synthesis inhibitors 1; DCB, 2,6-dichlorobenzonitrile; DMSO, dimethylsulfoxide; MS, Murashige–Skoog; ORF, open reading frame; PAW, phenol : acetic acid : water (2 : 1 : 1, w/v/v); RT–PCR, reverse transcription–PCR; SNP, single nucleotide polymorpholism; SuSy, sucrose synthase; TCA, trichloroacetic acid; TFA, trifluoroacetic acid; TLC, thin-layer chromatography; UDPG, UDP-glucose

Journal Article.  8970 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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