Journal Article

<i>n</i>-Butanol induces depolymerization of microtubules in vivo and in vitro

Ai Hirase, Takahiro Hamada, Tomohiko J. Itoh, Teruo Shimmen and Seiji Sonobe

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 47, issue 7, pages 1004-1009
Published in print July 2006 | ISSN: 0032-0781
Published online July 2006 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcj055
n-Butanol induces depolymerization of microtubules in vivo and in vitro

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The effects of butanol on microtubules (MTs) were examined by immunofluorescence microscopy. Fragmentation of cortical MTs was induced by n-butanol, but not by s- and t-butanols, in cultured tobacco BY-2 cells. Taxol prevented n-butanol-induced MT fragmentation. Fragmented cortical MTs were still attached to the inner face of the plasma membrane when n-butanol-treated protoplasts were ruptured on the slide glass. Moreover, MTs were depolymerized in the presence of n-butanol in vitro. Therefore, n-butanol is not only an activator of phospholipase D but also an effective MT-depolymerizing agent.

Journal Article.  2511 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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