Journal Article

Constitutive Accumulation of <i>cis</i>-piceid in Transgenic Arabidopsis Overexpressing a Sorghum Stilbene Synthase Gene

Christine K. Y. Yu, Corey N. W. Lam, Karin Springob, Jüergen Schmidt, Ivan K. Chu and Clive Lo

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 47, issue 7, pages 1017-1021
Published in print July 2006 | ISSN: 0032-0781
Published online July 2006 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcj061
Constitutive Accumulation of cis-piceid in Transgenic Arabidopsis Overexpressing a Sorghum Stilbene Synthase Gene

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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Sorghum SbSTS1 was the first example of a stilbene synthase gene in monocots. Previously, we demonstrated that the gene was involved in defense responses. To examine its biochemical function in planta, SbSTS1 was overexpressed in transgenic Arabidopsis. Metabolite analysis revealed that cis-resveratrol glucoside (piceid) accumulated as the major stilbene in the transgenic lines. Using liquid chromatography–tandem mass spectrometry (LC–MS/MS) in selected reaction monitoring mode, up to 580 μg g−1 FW of cis-piceid were detected in 2-week-old plants, which represent a convenient source of the cis-isomers for pharmacological investigations. Our results also suggested the presence of unknown stilbene isomerase activities in Arabidopsis.

Keywords: Arabidopsis; cis-resveratrol; cis-piceid; Sorghum; Stilbene synthase

Journal Article.  2683 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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