Journal Article

Purification and Characterization of Plant Dynamin from Tobacco BY-2 Cells

Takahiro Hamada, Hisako Igarashi, Maki Yao, Takashi Hashimoto, Teruo Shimmen and Seiji Sonobe

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 47, issue 8, pages 1175-1181
Published in print August 2006 | ISSN: 0032-0781
Published online August 2006 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcj086
Purification and Characterization of Plant Dynamin from Tobacco BY-2 Cells

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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We purified an 84 kDa polypeptide from the MAP (microtubule-associated protein) fraction of tobacco BY-2 cultured cells. LC/MS/MS (liquid chromatography–tandem mass spectrometry) analysis revealed that this polypeptide is a tobacco homolog of AtDRP3 (Arabidopsis thaliana dynamin-related protein 3). Electron microscopy revealed that NtDRP3 (Nicotiana tabacum dynamin-related protein 3) assembles to form a filamentous structure. When GDP was added to the NtDRP3 fraction, the filaments disappeared and many particles appeared. Biochemical analysis revealed that NtDRP3 could bind to and bundle both microtubules and actin filaments in vitro.

Keywords: Actin filament; Dynamin; Microtubule; Microtubule-associated protein (MAP); NtDRP3; Tobacco BY-2 cell

Journal Article.  2933 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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