Journal Article

Regulation of Nitrate Reductase by Non-Modifiable Derivatives of PII in the Cells of <i>Synechococcus elongatus</i> Strain PCC 7942

Nobuyuki Takatani, Masaki Kobayashi, Shin-ichi Maeda and Tatsuo Omata

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 47, issue 8, pages 1182-1186
Published in print August 2006 | ISSN: 0032-0781
Published online August 2006 | e-ISSN: 1471-9053 | DOI: https://dx.doi.org/10.1093/pcp/pcj088
Regulation of Nitrate Reductase by Non-Modifiable Derivatives of PII in the Cells of Synechococcus elongatus Strain PCC 7942

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In Synechococcus elongatus, the PII protein inhibits both transport and reduction of nitrate when ammonium is present in the medium. Using a transporter mutant having ammonium-resistant nitrate transport activity as the genetic background, we analyzed specific effects of PII on in vivo nitrate reductase activity by measuring uptake of nitrate from the medium. The results showed that the regulation of nitrate reductase does not require changes in the electric charge or size of the side chain at the phosphorylation site of PII. Phosphorylation of PII is thus unlikely to play a role in the regulation of nitrate reductase.

Keywords: Cyanobacterium; Nitrate assimilation; Nitrate reductase; PII protein; Post-translational regulation; Synechococcus elongatus strain PCC 7942

Journal Article.  2928 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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