Journal Article

Characterization of Cyanobacteriochrome TePixJ from a Thermophilic Cyanobacterium <i>Thermosynechococcus elongatus</i> Strain BP-1

Takami Ishizuka, Takashi Shimada, Koji Okajima, Shizue Yoshihara, Yuriko Ochiai, Mitsunori Katayama and Masahiko Ikeuchi

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 47, issue 9, pages 1251-1261
Published in print September 2006 | ISSN: 0032-0781
Published online September 2006 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcj095
Characterization of Cyanobacteriochrome TePixJ from a Thermophilic Cyanobacterium Thermosynechococcus elongatus Strain BP-1

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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A putative photoreceptor gene, TepixJ, of a thermophilic cyanobacterium is homologous to SypixJ1 that mediates positive phototaxis in the unicellular motile cyanobacterium Synechocystis sp. PCC 6803. The putative chromophore-binding GAF domain of TePixJ protein was overexpressed as a fusion with a polyhistidine tag (His-TePixJ_GAF) in Synechocystis cells and isolated to homogeneity. The photoreversible conversion of His-TePixJ_GAF showed peaks at 531, 341 and 266 nm for the green light-absorbing form (Pg form), and peaks at 433 and 287 nm for the blue light-absorbing form (Pb form). At 77K, the Pg form fluoresced at 580 nm, while the Pb form did not emit any fluorescence. Mass spectrometry of the tryptic chromopeptide demonstrated that a phycocyanobilin isomer binds to the conserved cysteine at ring A via a thioether bond. It is established that TePixJ and SyPixJ1 are novel photoreceptors in cyanobacteria (‘cyanobacteriochromes’) that are similar, but distinct from the phytochromes and bacteriophytochromes.

Keywords: Cyanobacteriochrome; GAF domain; Photoreceptor; Phototaxis; Phycocyanobilin; Phytochrome

Journal Article.  4985 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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