Journal Article

<i>Arabidopsis</i> Tubulin Folding Cofactor B Interacts with α-Tubulin In Vivo

Pankaj Dhonukshe, Bastiaan O.R. Bargmann and Theodorus W.J. Gadella

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 47, issue 10, pages 1406-1411
Published in print October 2006 | ISSN: 0032-0781
Published online October 2006 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcl001
Arabidopsis Tubulin Folding Cofactor B Interacts with α-Tubulin In Vivo

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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Microtubule biogenesis requires αβ tubulin dimers that are generated from α and β tubulin following post-translational modification by several tubulin folding cofactors (TFCs). Here we report the isolation and characterization of Arabidopsis TFCB (AtTFCB). AtTFCB is expressed in all organs of Arabidopsis. The subcellular localization of AtTFCB is mainly cytosolic. AtTFCB–overexpressing cells have fewer microtubules compared with the controls. Multimode fluorescence resonance energy transfer (FRET) microscopy reveals a direct physical interaction of AtTFCB with α tubulin in living plant cells. We conclude that AtTFCB interacts with α tubulin in vivo and its overexpression reduces the number of microtubules.

Keywords: Cofactor; FRET; GFP; Microtubule; Tubulin folding

Journal Article.  3196 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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